The p70 S6 Kinase (Ab-424) Antibody from MyBioSource.com is a Rabbit Polyclonal antibody to p70 S6k1, p70 S6KA, p70S6K, p70S6K1, RPS6KB1, S6K, and S6K1. This antibody recognizes Human, Mouse, and Rat antigen. The p70 S6 Kinase (Ab-424) Antibody has been validated for the following applications: Immunofluorescence, Immunohistochemistry, and Western Blot.
Description
RPS6KB1 phosphorylates the Ribosomal Protein-S6. Activation of RPS6KB1 requires a complex, ordered series of conformational changes and phosphorylation reactions. While the role of sequential, multi-site phosphorylation has been extensively detailed, characterization of the priming step required to initiate this cascade has remained elusive. Probably this priming process is dependent on calcium. Calcium-dependent regulation of RPS6KB1 does not specifically target Thr-229 and Thr-389, the key regulatory phosphorylation sites; rather, calcium chelation results in a global inhibition of RPS6KB1 phosphorylation. The initial calcium-dependent process is required to release an inhibitory interaction between the C- and N-termini of RPS6KB1, thus allowing phosphorylation of key domains. The priming event involves formation of a calcium-dependent protein complex that releases the interaction between the N- and C-termini. RPS6KB1 is then accessible for activation by the kinases that target the known regulatory phosphorylation sites